123rd General Meeting of the KCS

Type Poster Presentation
Area Analytical Chemistry
Room No. Exhibition Hall 2
Time 4월 19일 (금요일) 11:00~12:30
Code ANAL.P-283
Subject Implementation of LC-MS based proteomic analysis for investigating the tumorigenic roles of PAF in lung adenocarcinoma
Authors Jiwon Hong, Dowoon Nam, Jingi Bae, Seunghoon Back, Su-Jin Kim, Sang-Won Lee*
Department of Chemistry, Korea University, Korea
Abstract Lung adenocarcinoma (LUAD) is known to contribute as the most common category in lung cancer. Where proliferating cell nuclear antigen-associated factor (PAF) is associated with cell plasticity, cell stemness, cell hyperproliferation and gene transactivation, it is specifically expressed in tissue stem cells and cancer cells including LUAD. Nonetheless, its role in LUAD still remains to be unclear. Here, we plan to reveal the oncogenic roles of PAF in lung adenocarcinoma employing a comprehensive LC-MS based proteomic analyses on overall protein and phosphorylation changes. With the 6-plex TMT labeled peptides of the control and PAF knockdown cancer cell lines, quantitative profiling of global proteome and phosphoproteome were conducted for both human and mouse samples. Our profiling experiments resulted in identification of 306,938 distinct peptides of 11,689 protein groups and 21,974 distinct phosphopeptides of 17,822 phosphorylation sites for mouse cell lines, and 27,503 distinct peptides of 11,336 protein groups and 21,080 distinct phosphopeptides of 17,461 phosphorylation sites for human cell lines, respectively. From the currently identifying PAF knockdown-induced protein changes, phosphorylation changes and their associated pathways, we aim for a more comprehensive understanding of the pathogenic roles of PAF in the progression of LUAD.
E-mail jiwon_hong@korea.ac.kr