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|
| Type |
Poster Presentation |
| Area |
Analytical Chemistry |
| Room No. |
Exhibition Hall 2 |
| Time |
4월 19일 (금요일) 11:00~12:30 |
| Code |
ANAL.P-284 |
| Subject |
Development of extensive global proteome and phosphoproteome profiling platform for effective proteogenomic analysis on human cancer tissue |
| Authors |
Dowoon Nam, Sang-Won Lee*
Department of Chemistry, Korea University, Korea
|
| Abstract |
Mass spectrometry-based bottom-up proteomics is currently the leading method for large-scale proteome identification and quantification. Importance of proteomics can be highlighted by several aspects such as the lack of coverage that mRNA can precisely predict protein expression. Many of recent proteomic analysis provide increasingly extensive and deep proteome data due to the developments in mass spectrometry of high sensitivity and accuracy, effective fractionation, high-resolution separation and highly informative data analysis method. However, current proteomic data still suffers low in gene coverage compared to genomic data mainly because of the large dynamic range and complex PTMs of a proteome. The large discrepancy in gene coverage of proteomic and genomic data prevents an effective integration of the two data in proteogenomic studies. Here we report a novel dual-online noncontiguous fractionating and concatenating reverse-phase/reverse-phase liquid chromatography (DO-NCFC-RP/RPLC) technology employing online and its application for extensive proteome profiling. When combined with multiplexing capability of TMT and efficient enrichment of “one-pot” IMAC, the platform resulted in significantly improved analysis depth in phosphoproteome. |
| E-mail |
dowoon0517@korea.ac.kr |
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123rd General Meeting of the KCS