123rd General Meeting of the KCS

Type Poster Presentation
Area Polymer Chemistry
Room No. Exhibition Hall 2
Time 4월 19일 (금요일) 11:00~12:30
Code POLY.P-34
Subject Organic-Inorganic Nanocomposites based on Self-assembled, Fusion Protein Nanostructures for Theragnosis
Authors Min Jeong Kang, Aamna Basheer, Dong Woo Lim1,*
Department of Bionanotechnology, Hanyang University, Korea
1Department of Bionano Engineering, Hanyang University, Korea
Abstract Organic-inorganic nanocomposites based on protein nanostructures have been of growing interest for nanomedicine applications. In this study, we report self-assembled, fusion protein nanostructures and growth of metallic nanoparticles (MNPs) within them for theragnosis. The fusion proteins composed of penta-helices for self-assembly, thermally sensitive elastin-based polypeptide (EBP) and two different antiangiogenic peptides (AFs) for inhibition of angiogenesis were genetically engineered, expressed and purified by using non-chromatographic purification method. Furthermore, MNPs were grown within the protein nanostructures via metal ion binding and subsequent reduction for diagnosis of angiogenesis. Thermal-sensitivity and self-assembly of fusion proteins as well as MNP synthesis were characterized by UV-Vis absorbance, dynamic light scattering and transmission electron microscopy. This work suggests that fusion protein-based composite nanostructures would have great potential for theragnosis in angiogenesis-related diseases.
E-mail kmj910322@hanyang.ac.kr