|
Type |
Poster Presentation |
Area |
Polymer Chemistry |
Room No. |
Exhibition Hall 2 |
Time |
4월 19일 (금요일) 11:00~12:30 |
Code |
POLY.P-34 |
Subject |
Organic-Inorganic Nanocomposites based on Self-assembled, Fusion Protein Nanostructures for Theragnosis |
Authors |
Min Jeong Kang, Aamna Basheer, Dong Woo Lim1,* Department of Bionanotechnology, Hanyang University, Korea 1Department of Bionano Engineering, Hanyang University, Korea |
Abstract |
Organic-inorganic nanocomposites based on protein nanostructures have been of growing interest for nanomedicine applications. In this study, we report self-assembled, fusion protein nanostructures and growth of metallic nanoparticles (MNPs) within them for theragnosis. The fusion proteins composed of penta-helices for self-assembly, thermally sensitive elastin-based polypeptide (EBP) and two different antiangiogenic peptides (AFs) for inhibition of angiogenesis were genetically engineered, expressed and purified by using non-chromatographic purification method. Furthermore, MNPs were grown within the protein nanostructures via metal ion binding and subsequent reduction for diagnosis of angiogenesis. Thermal-sensitivity and self-assembly of fusion proteins as well as MNP synthesis were characterized by UV-Vis absorbance, dynamic light scattering and transmission electron microscopy. This work suggests that fusion protein-based composite nanostructures would have great potential for theragnosis in angiogenesis-related diseases. |
E-mail |
kmj910322@hanyang.ac.kr |
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