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|
| Type |
Poster Presentation |
| Area |
Material Chemistry |
| Room No. |
Exhibition Hall 2 |
| Time |
4월 19일 (금요일) 11:00~12:30 |
| Code |
MAT.P-481 |
| Subject |
Affinity-based Purification of Biopharmaceuticals Using Synthetic Binding Pairs |
| Authors |
An Jaeyeon, Junghyun Kim1, Kyeng Min Park1,*, Kimoon Kim*
Department of Chemistry, Pohang University of Science and Technology, Korea
1Center for Self-assembly and Complexity, Institute for Basic Science, Korea
|
| Abstract |
Biopharmaceuticals including monoclonal antibodies (mAb) and cytokines have occupied a major class of pharmaceuticals because of their remarkable efficacy compared to chemical drugs. Genetic engineering significantly contributed to high-level expression of therapeutic proteins in mammalian cells and bacteria for the mass production. However, multi-step purification through various chromatographies, mostly due to inefficient isolation of proteins of interest, reduces the efficiency of biomanufacturing. Although protein A-based affinity chromatography has been used for mAb purification, additional chromatographies are typically required for removal of undesired proteins having similar structural properties with the target mAb. In addition, this affinity chromatography cannot be used for other proteins including cytokines which do not have Fc region of immunoglobulins for protein A interaction. Here we report an efficient affinity-based purification method using synthetic binding pairs. The interaction between the binding pair is not affected by biomolecules such as proteins with sufficient enough binding affinity. Thus, this synthetic binding pair system can be a good alternative for purification of therapeutic proteins. |
| E-mail |
jeayoen@postech.ac.kr |
|
123rd General Meeting of the KCS