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|
| Type |
Poster Presentation |
| Area |
Life Chemistry |
| Room No. |
Exhibition Hall 2 |
| Time |
4월 19일 (금요일) 11:00~12:30 |
| Code |
LIFE.P-365 |
| Subject |
Structure, Dynamics, and Hyperthermostability of Acyl Carrier Protein from Thermotoga maritima |
| Authors |
Yeongjoon Lee, Yangmee Kim*
Department of Biotechnology, Konkuk University, Korea
|
| Abstract |
The acyl carrier protein from a hyperthermophile, Thermotoga maritima, was revealed to be extraordinary thermostable, allowing this protein to act as a carrier in the fatty acid synthesis (FAS) at extremely high temperature, higher than 80℃. To understand the key factors for the thermostability, the structure and dynamic features of the hyperthermophilic ACP (Tm-ACP) was investigated by NMR spectroscopy. The long α1α2 loop of Tm-ACP is stabilized by several hydrophilic and hydrophobic noncovalent interactions. Furthermore, an extensive ion cluster provides structural rigidity on the α2α3 loop, keeping hydrophobic cavity open and expanded compared to its mesophilic homologues. This important feature compensates the rigidity to shuttle acyl chains to FAS enzymes even at high temperature. |
| E-mail |
lyj7956@konkuk.ac.kr |
|
123rd General Meeting of the KCS