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|
| Type |
Poster Presentation |
| Area |
Life Chemistry |
| Room No. |
Exhibition Hall 2 |
| Time |
4월 19일 (금요일) 11:00~12:30 |
| Code |
LIFE.P-372 |
| Subject |
Amyloid fibril formation of fusion protein (ERLBD302-552-L23-αSyn) in the presence of ERLBD ligands |
| Authors |
Dawon Lee, Lee Kyunghee*
Department of Chemistry, Sejong University, Korea
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| Abstract |
In the brain of patients with Parkinson's disease, α-Synuclein (αSyn) protein forms amyloid fibrils. We previously examined the formation of αSyn amyloid in the presence of ERLBD ligand through ThT assay. In the present study, we studied the fibril formation of fusion proteins in which the ERLBD302-552 domain and αSyn were connected by a proper length of linker, L23. We successfully expressed and purified the fusion protein (ERLBD302-552-L23-αSyn) by affinity Ni2+-column purification. The amyloid formation process of fusion proteins was studied through ThT assay in the presence of ligands. Finally, photo-degradation of the fusion protein by the synthesized hybrid ligand was observed. |
| E-mail |
future0821@naver.com |
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123rd General Meeting of the KCS