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| Type |
Poster Presentation |
| Area |
Life Chemistry |
| Room No. |
Exhibition Hall 2 |
| Time |
4월 19일 (금요일) 11:00~12:30 |
| Code |
LIFE.P-375 |
| Subject |
Purification and identification anti-inflammatory peptides derived from tIK derivatives |
| Authors |
Hyunjun Jang, Yongae Kim*
Department of Chemistry, Hankuk University of Foreign Studies, Korea
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| Abstract |
RA is a severe autoimmune disease joint inflammation and the formation pannus tissue due to the synovial hyperplasia, which causes pro-inflammatory cells and macrophages for the destruction of cartilage and bone. Moreover, it is known that the disease is caused by an imbalance of pro-inflammatory cytokines and anti-inflammatory cytokines. But, recent studies have showed that truncated IK (tIK), which lacks the 315-amino acid sequence from the N - terminus, downregulates MHC class II on activation in inflammatory diseases. In our study, we examined the phosphorylation pattern of protein cell signaling by isolating macrophages from transgenic mice transplanted with the tIK nucleotide sequence, and found that tIK protein bound to the Interleukin 10 receptor subunit alpha (IL-10RA) and had the same effect as the anti-inflammatory cytokine IL-10 that plays a potent treatment by inhibiting immune cells and deactivating macrophages. Therefore, we focused on the process of finding derivatives that are shorter and better anti-inflammatory than the previously reported tIK protein. We predicted the possible structure of tIK based on IL-10 through sequence homology modeling that can calculate the distance between IL-10 and IL-10RA. Based on these results, we selected four epitopes that could have anti-inflammatory activity in tIK and selected the best anti-inflammatory peptide (tIK-YK4) using TH17 cell differentiation test. The peptide was re-truncated to 9-mer and 14-mer to confirm anti-inflammatory activity. Currently, we have successfully performed overexpression using E. coli and are optimizing the purification process. Finally, using purified peptides, we will identify the relationship between anti-inflammatory activity and structure. |
| E-mail |
zizidudgh1@naver.com |
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123rd General Meeting of the KCS