123rd General Meeting of the KCS

Type Poster Presentation
Area Medicinal Chemistry
Room No. Exhibition Hall 2
Time 4월 18일 (목요일) 11:00~12:30
Code MEDI.P-411
Subject Ginsenoside Rb2 suppresses the glutamate-mediated oxidative stress and neuronal cell death in HT22 cells
Authors Taejung Kim, Jiwoong Lim1, Heesu Lee2, Ae Nim Pae3, Jae Wook Lee4,*
Natural Products Research, Korea Institute of Science and Technology, Korea
1Convergence Research Center for Diagnosis, Treatme, Korea Institute of Science and Technology, Korea
2Department of Dentistry, Gangneung-Wonju National University, Korea
3Korea Institute of Science and Technology, Korea
4Convergence Research Center for Dementia DTC, Korea Institute of Science and Technology, Korea
Abstract Background: The objective of our study was to analyze the neuroprotective effects of ginsenoside derivatives, Rb1, Rb2, Rc, Rd, Rg1, and Rg3 against glutamate-mediated neurotoxicity in HT22 hippocampal mouse neuron cells. Methods: The neuroprotective effect of ginsenosides were evaluated by measuring cell viability. Protein expression of mitogen-activated protein kinase (MAPK), Bcl2, Bax, and apoptosis inducing factor (AIF) were determined by western blot analysis. The occurrence of apoptotic and death cells were determined by flow cytometry. Cellular level of Ca2+ and reactive oxygen spices (ROS) levels were evaluated by image analysis using the fluorescent probes Fluor-3 and 2’,7’-dichlorodihydrofluorescein diacetate (DCFDH), respectively. In vivo efficacy of neuroprotection was evaluated using the Mongolian gerbil of ischemic brain injury model. Result: Reduction of cell viability by glutamate (5 mM) was significantly suppressed by treatment with ginsenoside Rb2. Phosphorylation of MAPKs, Bax, and nuclear AIF was gradually increased by treatment with 5 mM of glutamate and decreased by co-treatment with Rb2. The occurrence of apoptotic cells was decreased by treatment with Rb2 (25.7 M). Cellular Ca2+ and ROS levels were decreased in the presence of Rb2, and in vivo data indicated that Rb2 treatment (10 mg/kg) significantly diminished the number of degenerated neurons. Conclusion: Our results suggest that Rb2 possesses neuroprotective properties that suppress glutamate-induced neurotoxicity. The molecular mechanism of Rb2 is by suppressing the MAPKs activity and AIF translocation.
E-mail jwlee5@kist.re.kr