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| Type |
Oral Presentation |
| Area |
Oral Presentation of Young Analytical Chemists I |
| Room No. |
Room 202 |
| Time |
THU 10:32-10:36 |
| Code |
ANAL1.O-24 |
| Subject |
Development of melamine specific bioreceptor using phage-display and their verification |
| Authors |
ChanYeong Park, Seung Hoon Back, Tae Jung Park*
Department of Chemistry, Chung-Ang University, Korea
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| Abstract |
Recently, concerns about safety in food have increased, because carcinogens or illegal hazardous substance are discovered to cheat its component contents. There are many kinds of dangerous reagents, we have investigated the melamine. Originally, it was mostly employed to synthesize melamine-formaldehyde resins, laminates, and plastics. However, some people added the melamine in dairy products. Since the quality evaluation of dairy products is conducted by measuring nitrogen contents. The ingested melamine can crystallize in human renal and induce acute nephrolithiasis and kidney injury. Thus, the detection of melamine is the momentous issue. To detect the melamine, advanced analytical equipment such as liquid and gas chromatography, and Raman spectroscopy were introduced, but they demand for the skilled person, high cost, and long time. Even though the optical detection methods, such as colorimetry and fluorescence, are also being developed as another detection method, it is difficult to specifically detect the melamine since they just recognize the structural features of melamine like aromatic ring or hydrogen bond. In this regard, we have developed specific bioreceptors that selectively bind to the melamine through the phage-display technology. The technique has been used to find out the new bio-receptors for the target such as cell-surface proteins, chemical hapten, etc. In this study, we have discovered melamine-specific binding peptides and evaluated their specificity and affinity against the melamine using graphene oxide quantum dot (GOQD)-Hg2+ quenching system. As a result, they showed the high attraction for melamine with a low level of detection limit and appear the probability as a detection sensor for the electrochemical and colorimetric analyses. |
| E-mail |
pcyzzing@naver.com |
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123rd General Meeting of the KCS