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| Type |
Oral Presentation |
| Area |
Oral Presentation of Young Analytical Chemists I |
| Room No. |
Room 202 |
| Time |
THU 10:20-10:24 |
| Code |
ANAL1.O-21 |
| Subject |
Novel zwitterionic fluorescent polythiophene sensor for E.coli. detection |
| Authors |
Jimin Shim, Salah Mahmoud Tawfik Ahmed, Yong-ill Lee*
Department of Chemistry, Changwon National University, Korea
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| Abstract |
We developed a novel zwitterionic fluorescent polythiophene with high sensitivity for the detection of gram-negative pathogenic bacteria. The sensing probe using the zwitterionic fluorescent polymer was constructed with three different water soluble thiophene monomers via oxidative polymerization reaction. The zwitterionic fluorescent polythiophene bound to bacteria through ionic complexes between anionic groups on the bacterial surface and cationic groups after 15 min incubation. This finding demonstrated that the fluorescence on system operated via changes in the hydrophilic and hydrophobic nature of the zwitterionic fluorescent polymer at pH (7.4), at a fixed polymer concentration. The limit of detection for Escherichia coli was found to be 0.033 cfu/mL with linear range from 1.15x108- 1.15x109 cfu/mL. With these encouraging results, it is expected that it would open revenues for promising applications of zwitterionic polythiophene in real samples.
Keywords : zwitterionic polythiophene, Gram-negative bacteria, E.coli, Fluorescence enhancement, Bacterial surface |
| E-mail |
bluesky2824@naver.com |
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123rd General Meeting of the KCS