123rd General Meeting of the KCS

Type Oral Presentation
Area Oral Presentation of Young Analytical Chemists II
Room No. Room 202
Time FRI 10:24-10:28
Code ANAL2.O-22
Subject SERS-based microdroplet system for high-throughput gradient analysis
Authors Jinhyeok Jeon, Kyoung Neon Kim1, Jaebum Choo1,*
Department of Bionano Technology, Hanyang University, Korea
1Departoemt of Chemistry, Chung-Ang University, Korea
Abstract Microfluidic technology has emerged as a highly efficient tool for the study of various chemical and biological reactions in last two decades. Recently, we reported that high-throughput biomarker detections can be achieved using a single phase gradient microfluidic channels together with surface-enhanced Raman scattering (SERS) detection. However, the deposition of nanoparticle aggregates on channel surfaces induces the “memory effect” in the continuous flow regime, thus affecting both sensitivity and reproducibility. To resolve this problem, a SERS-based gradient microdroplet system has been developed in this work. Herein, the sample stacking problem could be solved since two-phase liquid/liquid segmented flow reduces the memory effect. A serial dilution of a reagent could be also achieved in a stepwise manner using microfluidic concentration gradient generators. Therefore, desired concentrations of a reagent could be sequentially trapped by a small volume of droplets to prevent the sample stacking. We believe that this microdroplet system will be of significant utility in monitoring chemical and biological reactions for various concentrations of a reagent.
E-mail jeonjh1004@naver.com