123rd General Meeting of the KCS

Type Oral Presentation
Area Oral Presentation of Young Analytical Chemists II
Room No. Room 202
Time FRI 10:20-10:24
Code ANAL2.O-21
Subject Fabrication of PEGylated Au@Ag core-shell nanoparticles for SERS imaging of multiple biomarkers expressed on cancer cells
Authors Namhyun Choi, Jaejeung Dang1, Jaebum Choo1,*
Department of Bionano Technology, Hanyang University, Korea
1Department of Chemistry, Chung-Ang University, Korea
Abstract Implementation of the use of metal nanoparticles in cellular imaging has been recently garnered much attention. We recently developed a surface-enhanced Raman scattering (SERS)-based cellular imaging technique to detect cancer phenotype markers expressed on cell surfaces. Therein, hollow gold nanospheres (HGNs) have been used as SERS nano tags since HGNs strongly enhance the Raman signal intensity of individual nanoparticles by localized surface plasmon effects through the pin holes in the particle structures. However, this technique has some limitations including desorption of Raman reporter molecules and aggregation of HGNs in aqueous condition. To resolve these problems, polyethylene glycol (PEG)-ylated silver shell-gold hollow core nanoparticles (AgHGNs) have been fabricated and used as SERS nanotags in this work. By the silver shell encapsulation, the desorption problem of Raman reporter molecules could be solved. In addition, the SERS intensity of AgHGNs was greatly enhanced over HGNs. Then these particles were encapsulated with PEGs to make them stable in different pH, temperature and salt conditions. Finally, these PEGylated AgHGNs were used for the SERS imaging of three different tumor-associated biomarkers (CD44, EpCAM and ErbB2) expressed on breast cancer cells to confirm their multiple marker detection capability.
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