|
Type |
Oral Presentation |
Area |
Oral Presentation of Young Analytical Chemists II |
Room No. |
Room 202 |
Time |
FRI 10:16-10:20 |
Code |
ANAL2.O-20 |
Subject |
Highly sensitive molecular diagnostics using SERS-PCR for Bacillus anthracis detection |
Authors |
Yixuan Wu, Younju Joung1, Sohyun Park1, Jaebum Choo1,* Department of Chemistry, Chung-Ang University, China 1Department of Chemistry, Chung-Ang University, Korea |
Abstract |
The real-time polymerase chain reaction (QPCR) technique has been extensively used for the diagnosis of a wide range of infectious diseases. This technique found valuable clinical applications in the diagnosis of viral diseases but it suffers from several problems including time-consuming process and high fluorescent background noise. To resolve these problems, we developed a conceptually new surface-enhanced Raman scattering (SERS)-based PCR technique for its clinical applications. Due to its highly sensitive detection capability, it was possible to determine the concentration of target DNA in a short time by decreasing the number of PCR cycles. Consequently, SERS-PCR provides a high sensitivity and a short detection time over the conventional fluorescence-based QPCR. In the present study, we employed TaqMan-PCR to amplify a target gene, and then used the SERS detection technique for its highly sensitive detection. The combination of PCR with SERS proves to be effective for the rapid and sensitive detection of a low concentration of a target gene in a short time. |
E-mail |
wuonexuan@hotmail.com |
|