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| Type |
Oral Presentation |
| Area |
Oral Presentation for Young Material Chemists |
| Room No. |
Room 303 (Live Streaming) |
| Time |
TUE 09:30-09:45 |
| Code |
MAT.O-3 |
| Subject |
High-throughput evolution of near-infrared serotonin nanosensors |
| Authors |
Markita Landry*, Sanghwa Jeong1
Chemical and Biomolecular Engineering, University of California, Berkeley, United States
1Chemical and Biomolecular Engineering, University of California, Berkeley, Korea
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| Abstract |
Imaging neuromodulation with synthetic probes is an emerging technology for studying neurotransmission. However, most synthetic probes are developed through conjugation of fluorescent signal transducers to preexisting recognition moieties such as antibodies or receptors. We introduce a generic platform to evolve synthetic molecular recognition on the surface of near-infrared fluorescent single-wall carbon nanotube (SWCNT) signal transducers. We demonstrate evolution of molecular recognition toward neuromodulator serotonin generated from large libraries of ~6.9 × 1010 unique ssDNA sequences conjugated to SWCNTs. This probe is reversible and produces a ~200% fluorescence enhancement upon exposure to serotonin with a Kd = 6.3 μM, and shows selective responsivity over serotonin analogs, metabolites, and receptor-targeting drugs. Furthermore, this probe remains responsive and reversible upon repeat exposure to exogenous serotonin in the extracellular space of acute brain slices. Our results suggest that evolution of nanosensors could be generically implemented to develop other neuromodulator probes with synthetic molecular recognition. |
| E-mail |
sanghwa@berkeley.edu |
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125th General Meeting of the KCS