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  • 09월 06일 15시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제112회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Combinatorial assay with luciferase and gold nanoparticle for detection of agrichemicals

2013년 9월 5일 12시 44분 40초
BIO.P-601 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
10월 16일 (수요일) 16:00~19:00
저자 및
김명선, 김기욱1, 박태정1,*
중앙대학교 화학과 나노바이오화학연구실, Korea
1중앙대학교 화학과, Korea
Agrichemicals are important substances to boost agricultural productivity-increase crop yield in the modern society. However, the residues of agrichemicals could cause serious health problems for human even at very low concentrations. Conventional instrument-based methods are the accurate quantitative analysis, however, they must cost a lot of money and are bulky and time-consuming. Thus, it is important to induce a simple and sensitive method for on-site detection of agrichemical residues. The luciferase is able to detect various agrichemicals because it has an inhibition effect against agrichemicals on the luciferase activity. Luciferase requires a luciferin substrate, ATP, oxygen, and Mg2+ ion to produce bioluminescent light. The catalytic reaction of luciferase alters a luciferin substrate and ATP to oxyluciferin and AMP. Gold nanoparticles (AuNPs) have been used for applications in biosensors due to their biocompatibility, outstanding biophysical and visible spectroscopic properties. Metal ions change the color and UV/Vis absorbance of AuNPs, whereas, the stability of AuNPs is preserved by ATP from the effect of metal ions. Thus, in the presence of agrichemicals, the catalytic reaction of luciferase do not occur, and then AuNPs are remained with its stability and color by ATP. On the other hand, when the agrichemicals do not exist, AMP produced by catalytic reaction of luciferase makes their color-change of AuNPs because of their aggregation. This rapid, sensitive and easy method is able to detect many types of agrichemicals.