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  • 09월 08일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제116회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Single bubble micro extraction for analysis of chlorophenols coupled with capillary electrophoresis

2015년 8월 26일 13시 22분 47초
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목 09시 : 08분
분석화학 - Oral Presentation of Young Analytical Chemists I
저자 및
조성민, 이인기, 정두수*
서울대학교 화학부, Korea
In conventional headspace (HS) extraction, analytes evaporated from a sample donor phase in a sealed vessel can be enriched to an acceptor phase often in a liquid form or a sorbent coated on a fiber. The maximum enrichment factor is given by the ratio of the donor and acceptor volumes. Thus an acceptor of small volume is preferred and a single acceptor drop of small volume hanging to the capillary tip has been used in HS extraction for capillary electrophoresis (CE) [1]. To solve the stability problem of the hanging drop, we recently developed HS in-tube microextraction (ITME)/CE using a liquid plug inside a separation capillary as an acceptor in place of the drop [2]. Here, we present single bubble microextraction (SBME) technique coupled with CE, a simple but easy way of conducting HS extraction of a small volume sample for subsequent CE analysis. Instead of using a sealed vessel to prepare a HS, an bubble much smaller than 1 ?L was formed at the tip of a capillary by injecting a plug of air into the capillary containing an acceptor plug and ejecting the air plug after immersing the capillary into a sample donor. Then, SBME-CE was performed using the acceptor plug remained in the capillary. Due to the small volumes of the bubble and acceptor plug, it was possible even for a small volume donor to retain a high ratio of the donor and acceptor volumes for high enrichment factors. All steps of SBME-CE were carried out automatically using a commercial CE instrument without any modification. Therefore not only the sample volume could be dramatically reduced, but also the need for a special apparatus was eliminated.