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제120회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Investigation on The Stability of Uric Acid and Its Isotope (1,3-15N2) in Ammonium Hydroxide for The Absolute Quantification of Uric Acid in Human Serum

2017년 8월 31일 17시 00분 35초
ANAL.P-237 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
10월 19일 (목요일) 11:00~12:30
Analytical Chemistry
저자 및
Sun Young Lee, Young Eun Kim1, Kwonseong Kim2, Han Bin Oh2, Jongki Hong*, Dukjin Kang3,*
Department of Pharmacy, Kyung Hee University, Korea
1Metrology for Quality of Life Center for Bioanalys, Korea Research Institute of Standards and Science, Korea
2Department of Chemistry, Sogang University, Korea
3Metrology for Quality of Life, Korea Research Institute of Standards and Science, Korea
In clinical diagnosis, it’s well known that the abnormal level of uric acid (UA) in human body is implicated in diverse human diseases, for instance, chronic heart failure, gouty arthritis, diabetes, chronic renal failure, and kidney stones). In order to accurately diagnose UA-related diseases, the development of the method for the absolute quantification of UA in biological sample is required. As a primary method, an isotope dilution mass spectrometry (IDMS) has been used to obtain the accurate quantity of UA in blood or serum and also develop the certificated reference material (CRM) so as to provide a SI-traceability to clinical laboratories. Due to the low solubility of UA in water, an ammonium hydroxide (NH4OH) has been considered as a promising solvent to increase the solubility of UA and prepare both UA and its isotope standard solution for next IDMS-based absolute quantification. But, because of using this NH4OH solvent, it gives rise to the unwanted degradation of UA. In this study, we sought to optimize condition for the stability of UA in NH4OH solution by varying the mole ratios of UA to NH4OH at five different mole ratios of 1/1, 1/1.5, 1/2, 1/3, and 1/5.6, followed by LC-ID-MRM analysis. In addition, we also inspected minutely the effect of the storage temperatures [-20 ℃ and room temperature (RT)]. Finally, we performed the quantitative analysis of UA containing the KRISS serum certificated reference material (CRM, 111-01-02A) with diverse mixing ratios of UA to NH4OH and then compared those values to its certification value.