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제120회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Variation of separation efficiency of glycogen and pullulan with channel type in field-flow fractionation (FFF)

2017년 8월 24일 15시 58분 30초
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금 09시 : 50분
Analytical Chemistry - Oral Presentation of Young Analytical Chemists II
저자 및
Jaeyeong Choi, Catalina Sandra Fuentes Zenteno1, Mauricio Penarrieta2, Lars Nilsson1, Seungho LEE*
Department of Chemistry, Hannam University, Korea
1Department of Food Technology, Engineering and Nutrition, Lund University, Sweden
2Food Chemistry Group, Carrera de Ciencias Quimicas, Bolivia
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승인 3건
Field-flow fractionation (FFF) is a family of tools for the separation and characterization of particles and polymers. It has a broad dynamic range, and the utilization of an open channel requires no packing material, minimizing problems of sample adsorption, degradation and loss than in size exclusion chromatography (SEC). Frit inlet asymmetrical flow field-flow fractionation (FI-AsFlFFF) is a modified form of the asymmetrical field-flow fractionation (AsFlFFF) technique. Relatively higher carrier flow through the frit relative to the sample injection flow compresses the incoming sample band toward the accumulation wall of the channel. The main advantage of FI-AsFlFFF is to bypass the focusing step for relaxation in FlFFF that are normally carried out by stopping the carrier flow for a period of time to establish an equilibrium condition of the sample components. In this study, the separation efficiencies such as resolution, plate height and recovery were compared between AsFlFFF and FI-AsFlFFF channel using glycogen and pullulan. It was found that FI-AsFlFFF yields higher sample recovery than AsFlFFF. However, the resolution of FI-AsFlFFF was lower than that of AsFlFFF (about half). The resolution was increased with increasing the cross flow rate in both channels. Results also indicated that the resolution, plate height and the sample recovery changes with the type of samples. It seems a care needs to be taken in choosing the channel type in FFF studies.