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  • 09월 05일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제120회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Fast Direct Apolipoprotein E Genotyping for Alzheimer’s by Multi-channel Microchip Electrophoresis

2017년 8월 29일 16시 25분 25초
ANAL2.O-28 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
금 10시 : 15분
Analytical Chemistry - Oral Presentation of Young Analytical Chemists II
저자 및
Yucheng Sun, Nain Woo, Seong Ho Kang1,*
Department of Chemistry, Kyung Hee University, Korea
1Department of Applied Chemistry, Kyung Hee University, Korea
A direct apolipoprotein E (apoE) genotyping-based multi-channel microchip electrophoresis (ME) method was developed for fast high-throughput screening of Alzheimer. The target apoE polymorphism genes (i.e., 112 bp, 253 bp, 308 bp, 444 bp, and 514 bp DNA) were amplified by only 10 cycles of one-step multiplex polymerase chain reaction (PCR) from whole blood without DNA extraction. The amplified target DNA fragments were detected in 20 s by a programmed electric field strength (PEFS)-based single-channel ME. According to the optimum conditions of single-channel ME, target DNA fragments were separated using various multi-channel ME with three and five parallel channels, which simultaneously detected within 20 s without any loss of resolving power or reproducibility. The proposed multi-channel ME-PEFS assay technique provides a simple and accurate method for fast high-throughput screening of Alzheimer's disease, which can be simply apply to more large number of parallel channels for more high-throughput screening.