KCS

A direct apolipoprotein E (apoE) genotyping-based multi-channel microchip electrophoresis (ME) method was developed for fast high-throughput screening of Alzheimer. The target apoE polymorphism genes (i.e., 112 bp, 253 bp, 308 bp, 444 bp, and 514 bp DNA) were amplified by only 10 cycles of one-step multiplex polymerase chain reaction (PCR) from whole blood without DNA extraction. The amplified target DNA fragments were detected in 20 s by a programmed electric field strength (PEFS)-based single-channel ME. According to the optimum conditions of single-channel ME, target DNA fragments were separated using various multi-channel ME with three and five parallel channels, which simultaneously detected within 20 s without any loss of resolving power or reproducibility. The proposed multi-channel ME-PEFS assay technique provides a simple and accurate method for fast high-throughput screening of Alzheimer's disease, which can be simply apply to more large number of parallel channels for more high-throughput screening.