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제121회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Brain cytoplasmic 200 RNA in cancer cells

2018년 3월 8일 13시 51분 58초
BIO3-5 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
금 10시 : 20분
Life Chemistry - Current Trends in Biological Chemistry
저자 및
Younghoon Lee
Department of Chemistry, Korea Advanced Institute of Science and Technology, Korea
Brain cytoplasmic 200 RNA (BC200 RNA), a neuron-specific non-coding RNA, is also highly expressed in a number of tumors of non-neuronal origin. BC200 RNA is known to contribute to metastasis in several cancer cell lines, but the underlying mechanisms were not understood in detail. Furthermore, the biosynthesis of BC200 RNA remains poorly understood. We identified hnRNP E1 and hnRNP E2 as BC200 RNA-interacting proteins and found that these hnRNA proteins could restore translation inhibited by BC200 RNA in vitro. When BC200 RNA was knock-downed, cell migration and invasion were reduced, suggesting that BC200 RNA promotes cell motility. We obtained, from an antigen binding fragment (Fab) combinatorial phage library, a high affinity anti-BC200 RNA antibody that can be used for analysis of the RNA conformation in cells. Using this antibody, we found that BC200 RNA exists as two distinct forms (antibody-recognizable and nonrecognizable) in cancer cells and that their distribution depends on the cell type. We also analyzed biosynthesis of BC200 RNA. Efficient transcription of BC200 RNA requires both internal and upstream promoter elements. The cellular levels and half-lives of BC200 RNA were found to differ across various cancer cell types. BC200 RNA might be protected by some limiting factor(s) in cancer cells.