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09월 10일 16시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능
제124회 대한화학회 학술발표회, 총회 및 기기전시회 안내
NMR structural studies of tIK fragment with anti-inflammatory effective
2019년 8월 22일 16시 22분 18초
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금 09시 : 36분
Analytical Chemistry -
Oral Presentation of Young Analytical Chemists II
, Hyunjun Jang
, Ji-Ho Jeong
, Yongae Kim
Department of Chemistry, Hankuk University of Foreign Studies, Korea
Rheumatoid arthritis is caused by an autoimmune disorder. Various immune cells such as T cells, B cells, macrophages and dendritic cells participate in the inflammatory response of RA. Initially, inflammation starts around the synovial membrane around the joint, spreading around cartilage, ligaments, and destruction. In addition, inflammation of the blood vessels and skin may lead to systemic diseases such as vasculitis, anemia, and headache. There are many causes for the disease, but one of them is known as the imbalance of pro-inflammatory cytokines and anti-inflammatory cytokines. In addition, self-antigen recognition due to abnormal major histocompatibility complex (MHC) class II-expressing B cells produces antibodies that induce more severe RA. Recent studies have showed that truncated-IK (tIK) protein`s derivatives downregulate MHC class II on activation in inflammatory diseases. Therefore, we conducted epitope research to develop tIK as a new anti-inflammatory therapeutic agent for rheumatoid arthritis patients. In our study, we examined the phosphorylation pattern of protein cell signaling by isolating macrophages from transgenic mice transplanted with the tIK nucleotide sequence. It has been reported that tIK protein phosphorylates the 496th tyrosine of interleukin 10 receptor subunit alpha and has anti-inflammatory effect. We sought to find tIK protein’s specific regions that induce phosphorylation at the interleukin 10 receptor subunit alpha. So, we predicted the possible structure of tIK based on IL-10 through sequence homology modeling and proposed 4 anti-inflammatory peptide candidates and identified the anti-inflammatory activity through the TH17 cell differentiation test. Among them, the 18-mer peptide with anti-inflammatory activity was named tIK-YK4. Afterwards, we found 9-mer and 14-mer, based on 18-mer, and anti- inflammatory effect was also confirmed. Currently, we have successfully performed overexpression using E. coli and are optimizing the purification process. And we are trying to identify the relationship between structure of these peptides and anti-inflammatory activity through NMR studies.
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