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  • 09월 20일 16시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제126회 대한화학회 학술발표회 및 총회 Photothermal effect analysis of Bi-Te covered nanorods on 3D spheroid neuroblastoma cells

2020년 9월 10일 16시 30분 25초
ANAL1.O-15 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
화 09시 : 56분
Analytical Chemistry - Oral Presentation of Young Analytical Chemists I
저자 및
Chae ri Park, Chae Eun Heo, MyungKook Son, Dongjoon Im, Min Ji Kim, Sooyeon Chae, Hugh I. Kim*
Department of Chemistry, Korea University, Korea
Neuroblastoma is a solid tumor that almost always affects children. It starts in immature nerve cells found in an embryo or fetus. These days, nanoparticle for cancer treatment is being actively studied. The major function of these nanoparticles in therapy is drug delivery vesicles, imaging agents, and photothermal effect etc. So far, most studies of nanoparticle have been based on two-dimensional (2D) cell models. However, 2D cell models are hard to provide an accurate representation of the in vivo environment of the solid tumor. In this study, we use three-dimensional (3D) human neuroblastoma SK-N-SH cell spheroid models instead of 2D cell models to overcome the limitation of 2D cell models. After 5 days of culturing 3D spheroids, we treated Bi-Te covered nanorods (BTCN) that is previously reported for its photothermal and photoacoustic effect on 2D cancer cell models. BTCN is dissolved in serum free media and treated at spheroids with various concentrations for 4 hours. Then, 808nm 5 W/cm2 near IR laser light is irradiated for 2 minutes per one spheroid. After this process, we measured cell viability of the spheroids to identify the effect of BTCN with laser irradiation in 3D cell models. Also, we examine a form of the spheroids through a microscope, looked at the distribution of living cells and dead cells through confocal microscope. The results obtained from these techniques will help us to find out the difference between the therapeutic effects of BTCN in the 2D and 3D cell models, and how to increase their effectiveness in 3D cell models that is similar to reality.