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  • 09월 20일 16시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제126회 대한화학회 학술발표회 및 총회 Serum exosomes analysis differentiating patients of alcoholic and nonalcoholic fatty liver diseases

2020년 9월 9일 23시 46분 53초
ANAL2.O-11 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
화 10시 : 40분
Analytical Chemistry - Oral Presentation of Young Analytical Chemists II
저자 및
Huu-Quang Nguyen, Jaebeom Lee, Jeongkwon Kim*
Department of Chemistry, Chungnam National University, Korea
Exosomes contain many biological materials originated from the releasing cells, including proteins, lipids and RNAs, and are known to be involved in the communication and transferring of components between cells.
In this study, exosomes were enriched from blood serum of patients diagnosed with various states of liver diseases, using multiple centrifugation cycles at 40,000 x g. The enriched exosome samples were analyzed using liquid chromatography tandem mass spectrometry (LC-MS/MS) with Orbitrap mass analyzer. The proteomics data from Orbitrap LC-MS/MS measurements were quantified using MaxQuant software with label-free quantification workflow, followed by univariate and multivariate statistical analysis. By analyzing 24 samples of four different liver disease types, significant differences in protein expression between the diseases, including previously reported markers of liver diseases. Principal component analysis using significantly altered proteins identified from MaxQuant analysis of the LC-MS/MS data provided a confident differentiation between alcoholic and non-alcoholic fatty liver disease groups.
These results provide insight into the use of exosome analysis using mass spectrometry-based proteomics as a tool to assist in diagnosis and prognosis in clinical settings. The new findings open up new possibilities of using mass spectrometry for point-of-care clinical applications as well as for disease discovery and prevention purposes.