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  • 09월 20일 16시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제126회 대한화학회 학술발표회 및 총회 Limit of Detection for HPLC-Photodiode Array Detection is Comparable to the Refractive Index Detection for Monosaccharide Analysis.

2020년 9월 17일 16시 25분 39초
ANAL2.O-15 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
화 10시 : 56분
Analytical Chemistry - Oral Presentation of Young Analytical Chemists II
저자 및
Iqbal Jalaludin, Jeongkwon Kim*
Department of Chemistry, Chungnam National University, Korea
High-performance liquid chromatography (HPLC) is most commonly equipped with an ultraviolet (UV) absorbance detectors due to its easy-to-use and good stability qualities. One of the greatest limitations, however, is their failure to produce proper sensitivity for analytes missing a suitable UV chromophore. Refractive Index (RI) detection is indeed an alternate solution to satisfy the former's drawback, while knowing its limitation of using only the isocratic system for HPLC application. UV-absorbance detectors are often overlooked in sugar-related research due to the compounds’ low to non-existent UV absorption. However, there is a crucial need to use the gradient method for sugar analysis, since each compound has a unique elution time. In this analysis, we compared the two detection methods for small sugars, i.e. fructose, glucose, sucrose and maltose, to determine the detection limit (LOD). Using the shortest wavelength (190 nm) of our photodiode array detector, the small sugars can be detected. We took this advantage to compare the LOD of the two detection methods, and our preliminary results suggest that the two are comparable for the monosaccharide analysis. This finding shows that UV-absorbance detection can be extended to monosaccharide quantification for quality assurance purposes.