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  • 02월 23일 15시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

Carbohydrate microarrays as powerful tools in studies of carbohydrate-mediated biological processes

2009년 3월 3일 11시 30분 47초
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금 09시 : 40분
유기화학 - Oral Presentation for Young Organic Chemists
저자 및
Sungjin Park, Injae Shin
Department of Chemistry, Yonsei University, Seoul 120-749, Korea, Korea
Glycans in living organisms play a critical role in both normal physiological and detrimental disease processes through their interactions with proteins. Carbohydrate microarrays have become very powerful tools to elucidate the molecular basis of carbohydrate-recognition events in a high-throughput manner. In 2002, we and others reported a novel, carbohydrate microarray based technology for this purpose. We prepared carbohydrate microarrays by immobilizing maleimide-linked carbohydrates on thiol-derivatized glass slides and carried out lectin binding experiments by using these microarrays. In 2004, we developed a novel, efficient, chemoselective immobilization method for the fabrication of chemical microarrays which relies on the reaction of hydrazide-conjugated compounds with epoxides derivatized on solid surfaces. Although these two approaches can be used to efficiently immobilize modified carbohydrates on the surface, the required synthetic manipulations are sometimes difficult and time consuming. Therefore we have developed a simple and direct immobilization technique that involves a one-step, site-specific attachment of diverse unmodified glycans to the hydrazide-derivatized glass surface. To demonstrate the generality of this direct immobilization method, we examined its use for the construction of carbohydrate microarrays containing a variety of glycans. The results of protein and cell-binding experiments indicate that the glycan microarrays, prepared by using this methodology, are applicable to the rapid evaluation of glycan-mediated biomolecular interactions and the determination of quantitative binding affinities between carbohydrates and proteins.