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제108회 대한화학회 학술발표회, 총회 및 기기전시회 안내 2-Ethynylfluorenone-Labeled 2'-Deoxyuridine: Sequence-Specific Fluorescence Changes upon Duplex Formation

등록일
2011년 8월 1일 12시 41분 28초
접수번호
1022
발표코드
Ⅲ-ORGN.P-184 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 <발표Ⅲ>
발표형식
포스터
발표분야
유기화학
저자 및
공동저자
허주영, 황길태
경북대학교 화학과, Korea

Highly sequence selective DNA detections using molecular beacons (MBs) are important tools in biotechnological applications. A novel MB system comprising a quencher-free linear beacon with only a single fluorophore can discriminate between fully matched and single base-mismatched sequences. In order to develop other quencher-free linear beacons, we have designed a UFO nucleotide bearing a 2-ethynylfluorenone moiety covalently attached to the C-5 position of deoxyuridine. The fluorescence of UFO ODNs exhibited a dependence on the nature of its FBs. When the FBs were dT or dC, the total discrimination factors (relative area ratios of the fluorescence intensity between the perfectly matched and mismatched targets) for the duplex formations were high. In particular, the UFO DNA probe with dT-FBs behaves more sensitively than any other combinations of FBs. We also found that when a short strand (five-nucleotide), which is a completely natural strand containing a deoxyguanine (dG) nucleobase, was used as an internal quencher, the intrinsic fluorescence of a fluorescent DNA probe was reduced by 24%. These results suggest that this type of probe is an extremely powerful tool for the detection of single nucleotide alterations, and moreover, they exhibit highly dA-selective fluorescence emission.


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