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제108회 대한화학회 학술발표회, 총회 및 기기전시회 안내 The Derivazation of Cholesterol and Cholesterol Oxidation Products (COPs) for HPLC/UV-Vis

등록일
2011년 8월 1일 15시 51분 51초
접수번호
1295
발표코드
Ⅳ-ANAL.P-206 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 <발표Ⅳ>
발표형식
포스터
발표분야
분석화학
저자 및
공동저자
이진주, 마경나, 명승운
경기대학교 화학과, Korea
Free cholesterol is an unsaturated alcohol, susceptible to oxidation in the presence of light, oxygen and high storage temperature. This autooxidation involves a free-radical reaction, which leads to the formation of cholesterol oxidation products (COPs). COPs turned out to have a variety of potentially atherogenic, cytotoxic, mutagenic and possibly carcinogenic affects in humans. But, because HPLC/UV-Vis has a low sensitivity to cholesterol and COPs, GC/MS or LC-MS/MS have been used. In this study, the derivatization method which are sensitive and selective on HPLC/UV-Vis was developed. Derivatization of free cholesterol and COPs (25-hydroxycholesterol, 20α-hydroxycholesterol, 5,6α-epoxycholesterol, 5,6β-epoxycholesterol, cholestane-3β-5α-6β-triol) that differentiate from traditional methods, which are more complex derivatization procedure, was performed. The derivatized cholesterol and COPs showed the improved sensitivity and selectivity on HPLC/UV-Vis and also the increased ionization efficiency in LC-MS/MS.

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