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제108회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Mechanical properties of collagen fiber incorporated phospholipid vesicle

2011년 8월 1일 15시 54분 26초
Ⅰ-BIO.P-214 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
목 <발표Ⅰ>
저자 및
이두호, 박수진, 이기성1, 신종식2, 김희석, 신관우3
서강대학교 화학과, Korea
1세화고등학교, Korea
2광영고등학교, Korea
3서강대학교 화학과 및 바이오융합과정, Korea
A giant unilamellar vesicle(GUV) has received much attention recently because they, as a biological cell mimic in size and shape, can be utilized as a biological mimics. Up to now, many researchers have reported the dynamics and physical properties of GUV, for examples, dynamics of GUV with shear elasticity, or lipid phase behaviors of GUV, etc. However, most biological cells contain organelles and cytoskeletal network in their cytoplasm. Specifically, in case of cytoskeleton which supplies structure to cells, the fiber network plays a key role in gaining the tensile strength, stabilizing and forming the cellular structure. And it is not hard to expect that the physical properties of GUV will be greatly affected when a cellular vesicles contains complex sub-cellular structures (organelles and protein networks) in the cytoplasm. Therefore, the combination of collagen fiber, as representative of filament, in GUV ought to be studied. We introduced the collagen-mediated fibers into the cytoplasm, resulting in the fiber network in the inner volume of GUVs. We then could study how the mechanical/physical properties of GUV membranes were affected with the incorporation of interior-organelles and protein-cytoskeletal network. Micropipette aspiration was used to test mechanical strength of GUV composed of phosphatidylcholine lipids. To determine the role of sub-cellular structures, this study investigates the dynamics of vesicles with and without the incorporation of collagen fibers. Produced by increasing pipette pressurization, membrane tensions for lysis of single vesicles at room temperature were measured.