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제108회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Compact Hydroxylated Nanocrystals for Foerster Resonance Energy Transfer with Cy3 Organic Dyes

2011년 8월 1일 15시 55분 16초
Ⅱ-MAT.P-303 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
목 <발표Ⅱ>
저자 및
김용욱, 임성준, 윤혜주, 신승구
포항공과대학교 화학과, Korea
We report bright, compact, biocompatible, and nonspecific interaction-free hydroxylated semiconductor nanocrystals as a Foerster resonance energy transfer (FRET) donor for Cy3 organic dye. CdSe nanocrystals emitting at 508 nm were prepared by photochemical etching, and the ZnS shell was grown over photochemically-etched CdSe nanocrystals. Resulting CdSe/ZnS core/shell nanocrystals were converted to hydroxylated ones using 3-mercapto-1-propnaol and conjugated with aminated biomolecules using N,N′-disuccinimidyl carbonate (DSC). The emission wavelength of nanocrystal―bioconjuagtes was designed to overlap with the 515 nm band of Cy3. The nanocrystal―Cy3 FRET pair was applied to the detection of DNA hybridization on a DNA chip. Cy3-conjugated target DNA was immobilized on amine-functionalized glass slide using DSC, hybridized with various concentrations of biotinylated probe DNA, and then tagged with biotinylated nanocrystals via streptavidin. Both the emission spectra and time-resolved photoluminescence (PL) decay of the nanocrystal―Cy3 FRET pair on a DNA chip were obtained. The rate of energy transfer (kT) was determined from the time-resolved PL data for Cy3, and the FRET efficiency was calculated from kT. Nanocrystal-based DNA detection is 10 to 100 times more sensitive than Cy3-based DNA detection. Remarkably, nanocrystals enable the detection of 10 zmol DNA on a DNA chip without nonspecific interactions, demonstrating the efficiency of hydroxylated nanocrystals as a FRET donor to Cy3 organic dye. Furthermore, Cy3 excited by resonance energy transfer has much greater photochemical stability than Cy3 excited directly.