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학술발표회초록보기

초록문의 abstract@kcsnet.or.kr

결제문의 member@kcsnet.or.kr

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  • 08월 18일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제108회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Probing Transcriptional Dynamics in Living Bacterial Cells by Single-Molecule Fluorescence Imaging

등록일
2011년 8월 1일 16시 15분 53초
접수번호
1328
발표코드
PHYS2-6 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 11시 : 40분
발표형식
심포지엄
발표분야
물리화학 - Spectroscopic Approach to Biological Dynamics
저자 및
공동저자
이남기
포항공과대학교 시스템생명공학부/물리학, Korea
Recent advances in single-molecule fluorescence microscope techniques have allowed single-molecule sensitivity to probe various protein-DNA interactions, their structural changes, and fundamental cellular processes in a living cell. In this talk, I will introduce a novel single-molecule fluorescence microscope technique for in vivo single protein detection. To study transcription, the process of DNA-directed RNA biosynthesis in living cells, I used localization-enhancement fluorescence detection technique. I used a fluorescently tagged T7 RNA polymerase in bacteria containing a T7 promoter integrated into the chromosome. Since there are no other endogenous T7 promoters in the E. coli genome, localized T7 polymerase molecules correspond to transcription at the engineered promoter. In this way, I will demonstrate that it is possible to detect single RNA polymerase in living cells and directly measured promoter searching time, transcription initiation and elongation rates of T7 RNA polymerase. This work paves the way to observe individual protein machinery and to reveal fundamental cellular processes in living cells.

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