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  • 08월 18일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제108회 대한화학회 학술발표회, 총회 및 기기전시회 안내 A facile and efficient solidphase synthesis of linear peptide-based glycoclusters, Lectin Binding Propertie, and Detection of Carbohydrate-Binding Proteins in Cells

등록일
2011년 8월 2일 18시 44분 30초
접수번호
1422
발표코드
Ⅲ-ORGN.P-257 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 <발표Ⅲ>
발표형식
포스터
발표분야
유기화학
저자 및
공동저자
TIAN XIZHE, 배재영, 백경화, 고성균, 신인재
연세대학교 화학과, Korea
Abstract: A facile and efficient solidphase synthesis of linear peptide-based glycoclusters with various valences and different spatial arrangements of the sugar ligands is described. The synthetic strategy includes 1) solid-phase synthesis of fluorophore-labeled, alkynecontaining peptides, 2) coupling of azide-linked, unprotected mono-, di-, and trisaccharides to the alkyne-conjugated peptides on a solid support by click chemistry, and 3) release of the fluorophore-labeled glycoclusters from the solid support. By using this methodology, 32 fluorescent glycoclusters with a valence ranging from 1 to 4 and different spatial arrangements of the sugar ligands were prepared. Lectinbinding properties of the glycoclusters were initially examined by using microarrays immobilized by various lectins. These glycoclusters were then employed to detect the cell-surface carbohydrate-binding proteins in bacteria. Finally, the uptake of glycoclusters by mammalian cells through receptormediated endocytosis was evaluated. The results, obtained from the in vitro and in vivo studies, indicate that the binding affinities towar immobilized and cell-surface proteins are highly dependent on the valence and spatial arrangements of the sugar ligands in glycoclusters.

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