abs

학술발표회초록보기

초록문의 abstract@kcsnet.or.kr

결제문의 member@kcsnet.or.kr

현재 가능한 작업은 아래와 같습니다.
  • 08월 18일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제108회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Antioxidant effect of spermine is related to inhibition of matrix metalloproteinase

등록일
2011년 8월 5일 00시 29분 07초
접수번호
1501
발표코드
Ⅰ-BIO.P-231 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
목 <발표Ⅰ>
발표형식
포스터
발표분야
생명화학
저자 및
공동저자
채용병, 김문무1
동의대학교 화학과, Korea
1동의대학교 자연대학/화학과, Korea
The polyamines, putrescine, spermidine and spermine are low molecular weight cations present in many mammalian cells. The effect of spermine was investigated on antioxidant and matrix metalloproteinase-9 (MMP-9) in human colon cancer cells, SW480 cells. Thus, for the first time the direct scavenging effect of spermine on DPPH radical, superoxide anion, hydroxyl radical, hydrogen peroxide, reducing power and protective effect of DNA oxidation related to oxidative stress were evaluated in vitro. In addition, its inhibitory effects on the activity and expression of MMP-2 and -9 related to metastasis were determined using gelatin zymography. In this study, it was observed that spermine exhibited the highest inhibitory effect on hydrogen peroxide and hydroxyl radical in reactive oxygen species, and blocked DNA oxidation induced by hydroxyl radical. Moreover, it specifically inhibited both activity and expression of MMP-9 in SW480 cells. These results suggest that inhibitory effect of oxidative stress in the presence of spermine could inhibit the expression of MMP-9 in SW480 cells.

상단으로