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  • 08월 18일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제108회 대한화학회 학술발표회, 총회 및 기기전시회 안내 NMR Sutdy on Adenyl kinase(AK1) in Human

2011년 8월 5일 16시 19분 16초
Ⅰ-BIO.P-234 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
목 <발표Ⅰ>
저자 및
최현주, 우선희, 원호식
한양대학교 응용화학과, Korea
Adenylate Kinase (AK) is the enzyme presented in all living organisms from prokaryotes to eukaryotes. This enzyme helps transitions of phosphate group between ATP and AMP. Furthermore, it plays an important role in the synthesis of nucleic acid and energy metabolism. It has revealed that AK has more than seven isonzyme’s form. Each of these AK has patterns such as tissue-specific and organelle-specific. .AK isozyme present in the cytoplasm has a short form which is known to have greater activity than AK has a long form in mitochondria. Of AK isozymes, AK1 is expressed in all tissues such as liver, brain, heart, kidney and skeletal muscle and has about 200 amino acids. AK1 is consists of approximately 200 amino acids, whose research has been in progress.for a long time. However, research on NMR structure of Human AK1 and ATP, AMP binding sites is not yet known clearly. Therefore, We used samples that AK1 proteins isotope exchanged to C13, N15 and conducted 3D NMR experiments(HNCA, HNCO) using 800MHz Bruker NMR. Through this data we are able to make backbone assign. Studies on the Binding site residues is conducted by ATP and AMP titration experiments. In addition to doing experiments about T1, T2 relaxation and NOE we can obtain the molecular dynamics information. Finally we are planning to compare with AK1R138A which is substituted that 138 amino acids of the AK1 from Arginine to and Alanine. When this amino acid substituted , AK1 activity was significantly reduced. Thus, To know the reason for this phenomenon we are performing research on AK1R138A through the process of cloning AK1.