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  • 08월 18일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제108회 대한화학회 학술발표회, 총회 및 기기전시회 안내 The kinetic of in vitro synaptic vesicle fusion studied by diffusion-based single vesicle fusion assay

등록일
2011년 8월 5일 18시 12분 01초
접수번호
1627
발표코드
Ⅰ-BIO.P-237 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
목 <발표Ⅰ>
발표형식
포스터
발표분야
생명화학
저자 및
공동저자
최봉규, 김재열1
포항공과대학교 I-BIO/시스템생명공학부, Korea
1포항공과대학교 물리학과, Korea
Neurotransmission was conducted by membrane fusion between plasma membrane and synaptic vesicles containing neurotransmitter such as serotonin dopamine and acetylcholine. People believed that SNARE protein mainly induce the membrane fusion process. Effective regulation of function of SNARE proteins is needed for appropriate signal transduction. Synaptotagmin, especially, is a membrane protein that regulates membrane docking and fusion by interacting with SNARE proteins. Most people believed that synaptotagmin is a Ca2+-sensor for membrane fusion because of its specific Ca2+-binding affinity. but detail function of synaptotagmin is still elusive. In this work, we studied the molecular function of synaptotagmin in vesicle docking and fusion. Here, we used reconstituted vesicles in vitro and a single-molecule technique, alternating-laser excitation (ALEX). Through this approach, we can sort the multiple states of vesicles, docking and fusion, respectively. Our results suggest that synaptotagmin enhance the docking events and it leads to stimulation of whole membrane fusion process.

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