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제109회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Modular Aptasensor System Using Cucurbit[7]uril-Ferrocene Derivative As a Supramolecular Linker

2012년 2월 16일 16시 39분 37초
BIO.P-691 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
4월 25일 (수요일) 18:00~21:00
저자 및
이돈욱, 공보경, 류성호1, 김기문
포항공과대학교 지능초분자연구단, WCU 첨단재료과학부, 화학과, Korea
1포항공과대학교 분자생명과학부, Korea
Aptamers are artificial single-stranded nucleotide sequences that fold into secondary and tertiary structures making them bind to certain targets with extremely high specificity. Several advantages of aptamers over antibodies including high stability and easily modifiable structure make them promising sensing element for biosensor. However, only a few methods for immobilizing the aptamers on a sensor chip have been developed including the ones using the streptavidin-biotin pair, which has yet several demerits such as tedious immobilizing steps and limited stability under harsh conditions. Here we present our efforts to develop a surface plasmon resonance (SPR) sensor for specific protein detection using aptamer as a sensing unit and cucurbit[7]uril-ferrocene derivative (CB[7]-AFc) pair as a novel synthetic non-covalent linker for immobilizing aptamers. The 37 kDa protein thrombin and 15-mer oligonucleotide thrombin binding aptamer (TBA) were chosen as a model target protein and aptamer, respectively, to demonstrate the sensing ability of the system. The monolayer of synthetic receptor CB[7] on a gold surface produced by spontaneous adsorption successfully immobilized AFc-labeled TBA by ultrastable host-guest interaction between CB[7] and AFc. The immobilized TBA selectively sensed the injected thrombin in the concentration range 10-9 ? 10-7 M. The simple and modular method for surface immobilization using the synthetic receptor-ligand pair may broaden the applications of aptamer-based sensor.