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제109회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Optimal design of scalable photo-bioreactor and strain improvement for photoautotrophic culture of Haematococcus pluvialis

등록일
2012년 2월 20일 15시 27분 00초
접수번호
1296
발표코드
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발표시간
목 15시 : 45분
발표형식
심포지엄
발표분야
환경에너지 - Biomass-based energy and biomaterials
저자 및
공동저자
최승필
고려대학교 화공생명공학과, Korea
In photoautotrophic culture of Haematococcus pluvialis, both a development of photobioreactor and a strain improvement are important for the economic astaxanthin production. An low-energy multi-tubular photobioreactor were optimized in terms of performance factors, such as a slope of a V-shaped bottom design, a volumetric flow rate of air, a ratio of height/diameter and diameter of an air sparger. Consequently, through the optimization of photobioreactor, 2.62 g/L of the biomass density and 78.37 mg/L of astaxanthin concentration were obtained. In order to shorten the astaxanthin induction time, highly photoinducible mutant in photoautotrophic condition was made by enhancing inherent photosensitivity of Haematococcus cells using conventional random mutagenesis and well-defined isolation method (PS-PP). It was observed that the enhanced light-sensitive phenotype was associated with a partial lesion in photosystem II and elicited an improved astaxanthin production from an effective encystment and carotenogenesis. As a result, the concentrations of cell biomass and astaxanthin were dramatically increased by 19% and 64% under strong light and 40% and 126% under moderate light, respectively. These two developments will improve the yields of astaxanthin in photoautotrophic culture system and lead to economic production of astaxanthin using H. pluvialis.

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