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학술발표회초록보기

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  • 03월 02일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제109회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Highly sensitive mercury detection based on aptamer-thrombin interaction and enzymatic reaction

등록일
2012년 2월 21일 21시 17분 16초
접수번호
1316
발표코드
ELEC.P-1292 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
4월 25일 (수요일) 18:00~21:00
발표형식
포스터
발표분야
전기화학
저자 및
공동저자
고현준, 김규원
인천대학교 화학과, Korea
We present a highly sensitive detection of mercury(II) ions using thrombin and thrombin aptamer. Thrombin is a protein that plays many roles in the coagulation cascade-converting soluble fibrinogen into insoluble strands of fibrin as well as catalyzing many other coagulation-related reactions. Aptamers are oligonucleic acid or peptide molecules that bind to a specific target molecule. Aptamers are usually created by selecting them from a large random sequence pool. The aptamer folds into three-dimensional structures upon the exposure to target protein, which allow them to bind a specific protein. As a thrombin aptamer short single-stranded oligonucleotide including thymine (T)?rich sequence was employed in this study. Exposure of thrombin aptamer-modified surface to mercury ion(II) converts flexible aptamer into hairpin structure due to the formation of T-Hg2+-T pairs, which makes it difficult for thrombin to interact with aptamer. The estimation of the quantity of thrombin bound on the resulting surface will be demonstrated by using various methods such as cyclic voltammetry, electrochemcial impedance spectroscopy, surface plasmon resonance spectroscopy, and coagulation reaction of thrombin.

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