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  • 03월 02일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제109회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Molecular dynamics of synthetic chromophore derivatives of green fluorescent protein (GFP)

등록일
2012년 2월 22일 11시 22분 00초
접수번호
1328
발표코드
PHYS.O-5 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 10시 : 40분
발표형식
구두발표
발표분야
물리화학 - General Oral Presentation
저자 및
공동저자
이정화, Pi-Tai Chou1, 주태하
포항공과대학교 화학과, Korea
1National Taiwan University, Taiwan
The green fluorescence protein (GFP) has been widely used as biological markers in living cells, since it is noninvasive and does not need any substrate or coenzyme to fluoresce. The cyclization and oxidation reaction among three amino acids yield the fluorophore of GFP (p-HBDI). The chromophore without protein matrix shows low quantum yield of fluorescence which can be understood as conformational relaxation leading to radiationless deactivation. For suppression of conformational relaxation, o-HBDI was synthesized as an ortho isomer of p-HBDI. Earlier study revealed that o-HBDI undergoes ESIPT reaction with ultrafast rate. Various derivatives of o-HBDI, which have different functional groups like -Br, -NO2, -OMe and -phenyl, are synthesized to clarify the effect of the electronic property to ESIPT reaction. Also the photophysical property results in a wide spectral range of fluorescence, which gives feasibility of application like OLED. The time-resolved fluorescence measurement with high time resolution will unveil the ESIPT dynamics of derivatives of o-HBDI, about 30 fs. The ultrafast proton transfer can initiate coherent nuclear wavepacket motion in the product keto isomer, which is manifested by the oscillations of the time-resolved signals. Since there are dissimilarity of the vibrational frequencies in spite of the similarity of skeletal geometry, these TRF results are remarkable. We have performed quantum mechanical calculations to understand the origin of oscillatory components appeared in time resolved signals. This theoretical verification can figure out the proper structural change during the reaction and prove the possibility of TRF spectroscopy as an observer which collects the geometrical information of reaction dynamics.

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