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학술발표회초록보기

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  • 03월 02일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제109회 대한화학회 학술발표회, 총회 및 기기전시회 안내 The Effective Analysis of Gibberellic acid (GA3) using HPLC/UV-vis

등록일
2012년 2월 22일 16시 47분 43초
접수번호
1355
발표코드
ANAL.P-625 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
4월 25일 (수요일) 18:00~21:00
발표형식
포스터
발표분야
분석화학
저자 및
공동저자
마경나, 명승운1
경기대학교 자연과학부 화학과, Korea
1경기대학교 화학과, Korea
Gibberellins (GAs) are a class of plant growth hormones that exert profound and diverse effects on plant growth and development such as seed germination, fruit external ripening and fruit development and others. GAs are increasingly applied in agriculture to improve crop quality and yields. But, there is no regulation of GAs in Korea. On the other hand, the MRL(Maximum residual limits)s of Gibberllic acid (GA3) in Japan and EU are 200 μg/kg and 500 μg/kg, respectively. Determination of GA3 by HPLC/UV-vis has always been extremely difficult because GA3 has little ultraviolet (UV) absorbance. Therefore, in this study, the analytical method, which is sensitive and selective on HPLC/UV-vis, was developed. Derivatization of GA3 with benzyl alcohol was performed and the derivatized GA3 was effectively analyzed by HPLC/UV-vis. The HPLC/UV-vis was equipped with C8 column (4.6 mm I.D.× 150 mm, 3.5 μm) using a binary solvent system composed of containing 0.1% formic acid water and acetonitrile and flow rate was 0.8 mL/min. The established method can be applied to more effective analysis of GA3 from plant and food.

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