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제109회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Derivatization of Cholesterol and Cholesterol Oxidation Products (COPs) for HPLC/UV-Vis Analysis

등록일
2012년 2월 22일 16시 47분 55초
접수번호
1356
발표코드
ANAL.P-626 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
4월 25일 (수요일) 18:00~21:00
발표형식
포스터
발표분야
분석화학
저자 및
공동저자
이진주, 조현우, 명승운
경기대학교 화학과, Korea
Free cholesterol is an unsaturated alcohol susceptible to oxidation in the presence of light, oxygen and high storage temperature. This autooxidation involves a free-radical reaction which leads to the formation of cholesterol oxidation products (COPs). COPs turned out to have a variety of potentially atherogenic, cytotoxic, mutagenic and possibly carcinogenic affects in humans. Nevertheless, HPLC/UV-Vis are not sensitive enough to separate and quantify free cholesterol and COPs simultaneously. In this study, significant improvements are realized by increasing the UV absorption of compounds through derivatization attaching a chromophore to the functional groups of cholesterols. Effective and sensitive derivatization of free cholesterol and COPs (25-hydroxycholesterol, 7-ketocholesterol, 5,6α-epoxycholesterol, 5,6β-epoxycholesterol, cholestane-3β-5α-6β-triol) that differentiate from traditional methods, which are more complex derivatization procedure, was performed. The devatized cholesterol and COPs were separated simultaneously on a C18 column (2.1×100 mm, 3.5 μm) using a mobile phase of water and acetonitrile. The derivatized cholesterol and COPs showed the increased sensitivity and selectivity on HPLC/UV-Vis.

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