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제109회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Spectrofluorometric Determination of Bovine Serum Albumin by Enhanced Fluorescecne of Terbium Nanoparticles

등록일
2012년 2월 22일 16시 57분 16초
접수번호
1358
발표코드
ANAL.P-628 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
4월 25일 (수요일) 18:00~21:00
발표형식
포스터
발표분야
분석화학
저자 및
공동저자
ALAM MD AL MAHMNUR, MD.KAMRUZZAMAN, 이상학1
경북대학교 화학과, Bangladesh
1경북대학교 화학과, Korea
A new spectrofluorometric method has been developed for the determination of bovine serum albumin (BSA) using terbium nanoparticles (TbNPs) as fluorescence (FL) probe. TbNPs was synthesized by tannic acid reduction method and stablilized by sodium dodecyl benzene sulfonate (SDBS). The average size of the nanoparticles was found as 70-80 nm in transmission electron microscope (TEM). TbNPs emits maximum FL emission at the wavelength of 402 nm upon excitaion at 225 nm. When BSA was added to the colloidal solution of TbNPs, a remarkable enhancement of emission spectum was observed at the emission wavelength. Under the optimized condition, the FL emission intensity of the TbNPs-BSA system exhibited a linear correlation with the BSA concentration. Linearity was observed in the range of 4-120 nM of BSA with a limit of detection (LOD) of 0.76 nM. The precision of the method was tested at the concentration level of 1×10-8 M for 11 replicate measurements giving relative standard deviation (RSD) values of 1.25 %.

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