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학술발표회초록보기

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  • 03월 02일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제109회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Detection of biomarker protein OjaMT using Single Chain Variable Fragment antibodies

등록일
2012년 2월 22일 18시 41분 54초
접수번호
1365
발표코드
ANAL.P-630 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
4월 25일 (수요일) 18:00~21:00
발표형식
포스터
발표분야
분석화학
저자 및
공동저자
정덕호, 민경미, 권영은
동국대학교 의생명공학과, Korea
The expression of metallothionein in O.javanicus (OjaMT) increases as a result of heavy metal exposure. Measuring the level of OjaMT expression in O.javanicus can be a good monitoring method for heavy metal contamination of environment and their toxicity. Previously, western blotting and ELISA methods were used to monitor the level of OjaMT expression and these methods requires long detection time and gives high level of background. Also, it was rather difficult to achieve quantitative measurement of OjaMT. Here, we set-up an assay to quantitatively detect OjaMT using surface plasmon resonance (SPR) system. We have used OjaMT specific antibody for the SPR based label free detection of OjaMT. Also, we have constructed OjaMT specific scFv using the total RNA of antibody generating hybridoma cells using jumping PCR. The generated scFv’s exhibited specific binding activity to OjaMT in solution. ScFv’s were immobilized to gold substrate using a 3-Cys tag introduced during the cloning vector construction. The performance of two different probes were compared and the amount of OjaMT was measured quantitatively.

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