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제109회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Inhibitors of β-Ketoacyl acyl carrier protein synthase (KAS) III as Broad-spectrum Antimicrobial Agents

등록일
2012년 2월 22일 19시 57분 02초
접수번호
1370
발표코드
PHYS.P-507 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
4월 25일 (수요일) 18:00~21:00
발표형식
포스터
발표분야
물리화학
저자 및
공동저자
정기웅, 강동일1, 김양미
건국대학교 생명공학과, Korea
1건국대학교 화학과, Korea
β-Ketoacyl acyl carrier protein synthase (KAS) III is a particularly attractive target for development of antibacterial drug in the type II fatty acid synthetic pathway, since it is central to the initiation of fatty acid synthesis. We performed in silico screening in order to find inhibitors of Escherichia coli KAS III (ecKAS III), Staphylococcus aureus KAS III (saKAS III) and Enterococcus faecalis KAS III (efKAS III). Binding study of selected compounds from in silico screening was performed by Saturation Transfer Difference NMR spectroscopy and fluorescence experiments, and antimicrobial effect was evaluated against various bacteria. From this study, we found several potent inhibitors of ecKAS III, saKAS III and efKAS III which showed an antimicrobial effect against E. coli, S. aurues and E. faecalis as well as multidrug-resistant bacteria and we confirmed the importance of KAS III as targets for antimicrobial agents. Studies on the interactions between malonyl-ACP and ecKAS III showed that large chemical shift perturbations were observed for negatively charged residues in second helix of malonyl-ACP, suggesting that the electrostatic interactions are important for elongation reactions of acyl chains.

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