abs

학술발표회초록보기

초록문의 abstract@kcsnet.or.kr

결제문의 member@kcsnet.or.kr

현재 가능한 작업은 아래와 같습니다.
  • 03월 02일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제109회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Graphene oxide based enzyme platform for drug screening

등록일
2012년 2월 23일 11시 36분 40초
접수번호
1412
발표코드
ANAL.P-635 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
4월 25일 (수요일) 18:00~21:00
발표형식
포스터
발표분야
분석화학
저자 및
공동저자
장홍제, 유수윤, 민달희1
KAIST 화학과, Korea
1서울대학교 화학과, Korea
Helicases are a class of essential enzymes that unwind double stranded into single stranded nucleic acid. The unwinding of DNA is a key reaction for all single stranded DNA required processes such as cell proliferation and viral replication. Due to their critical role in viral process, helicases have been targeted for therapy in many virus diseases. Therefore, it is important to develop a simple, fast and cost-effective platform for helicase unwinding activity assay for diagnostic and drug discovery. We developed graphene oxide (GO) based helicase activity assay using flurescence on-off platform. The strategy relies on preferential binding of GO to single stranded DNA over double stranded DNA, thereby quenching fluorescence of dye labeled single stranded DNA. Our GO based strategy enabled real-time monitoring, quantitative and high-throughput assay of DNA unwinding activity. We found SARS and HCV helicase inhibiting hit compounds by using our GO based helicase assay platform.

상단으로