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  • 03월 02일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제109회 대한화학회 학술발표회, 총회 및 기기전시회 안내 microRNA detection platform inside living cells based on nanomaterials

2012년 2월 23일 11시 37분 52초
ANAL.P-636 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
4월 25일 (수요일) 18:00~21:00
저자 및
유수윤, 나희경, 민달희1
KAIST 화학과, Korea
1서울대학교 화학과, Korea
As biomolecules inside cells including proteins, nucleic acids, and small molecules show a variety of expression level and pattern, localization or distribution, they are considered as the critical parameters that reflect the state of organism including cellular behaviors, function, proliferation, development, physiological and pathological states. In that point of view, the build-up of the biomolecule detection method is one of the important issues in the biomedical field for the treatment process of all sorts of diseases. MicroRNAs (miRNAs) are a class of small-sizes (10~25nt) and non-coding RNA molecules that play an important regulatory role in the expression of diverse genes. Interestingly, miRNAs as the attractive biomolecule have been paid great attentions in a wide range of biological processes like development and metabolism and pathological progresses of disease/ disorders. Here, we fabricated the microRNA analytical platform for rapid, simple, and sensitive detection using nano-sized graphene oxide and detection probe antisense oligonucleotide for this study. We evaluated that 1) the probe-nanographene complex can work as microRNA sensing platform with efficient fluorescence quenching and recovery ability, 2) nanographene oxide sheets can serve as a delivery carrier of detection probe into live cells for real-time monitoring and quantitative analysis of microRNA and 3) the nanogrpahene oxide sheets provide the stable loading platform in the complex biological solutions and samples.