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학술발표회초록보기

초록문의 abstract@kcsnet.or.kr

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  • 03월 02일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제109회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Site-specific labeling of cell surface proteins based on protein trans-splicing

등록일
2012년 2월 23일 18시 21분 27초
접수번호
1490
발표코드
KCS2-5 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
목 10시 : 30분
발표형식
심포지엄
발표분야
Developing Bonds Between Women Chemists in the U.S. and Korea
저자 및
공동저자
권영은
동국대학교 의생명공학과, Korea
It is important to visualize movemont of proteins in live cell without interfereing their original function. Here we describe a highly specific and robust method for labeling cell surface proteins with synthetic probes. The method uses the trans-splicing reaction of Nostoc. punctiforme (Npu) DnaE split-intein. The trans-splicing reaction is a self-processing reaction and can be used for introduction of various synthetic probes to target proteins with a minimal size linker. In this work, we utilized cell surface proteins fused to N-terminal fragment of Npu. DnaE Intein and C-terminal fragment of Npu. DnaE Intein fused to a fluorescent probe. The expressed model proteins were successfully labeled with synthetic fluorescent probes through specific interaction between N- and C-fragment of inteins within 30 mins. No external energy was required for the labeling reaction. This improved labeling method will provide a useful tool for studying functions and mobilities of proteins in live cells.

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