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학술발표회초록보기

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  • 03월 02일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제109회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Application of proteomic analysis using trypsin coated magnetic nanofiber in rigorous conditions

등록일
2012년 3월 1일 15시 27분 46초
접수번호
1548
발표코드
ANAL.P-644 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
4월 25일 (수요일) 18:00~21:00
발표형식
포스터
발표분야
분석화학
저자 및
공동저자
박현주, 이상원
고려대학교 화학과, Korea
Developments of stabilization of proteases such as trypsin are needed for efficient protein digestion in proteomic analysis. In the present work, trypsin was stabilized in the form of enzyme coating on magnetic nano?ber (EC-TR/NP-NF), which crosslinks additional trypsin molecules onto covalently attached trypsin on magnetic nano?ber (CA-TR/NP-NF). EC-TR/NP-NF showed better stability than CA-TR/NP as well as free trypsin in rigorous conditions, such as at high temperatures of 40 and 50℃, in the presence of organic co-solvents, various pH’s, and super acoustic condition in digesting a model protein, enolase. Covalent linkages among trypsin molecules improved the stability of EC-TR/NP-NF and inhibits the denaturation, autolysis, and leaching of trypsin. Magnetic nanofiber which has magnetic property can be applied in reuses of protease for protein digestion. In rapid proteolytic digestion using super acoustic technology, EC-TR/NP-NF showed better performance and stable repeated digestion under 5 times reuses. The enzyme coating approach will be successfully employed not only for high throughput protein digestion in proteomic analysis but also for various other ?elds such as food processing and peptide synthesis.

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