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  • 03월 02일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제109회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Two-Photon Probes for Biomedial Applications

2012년 3월 8일 14시 42분 37초
ORGN1-6 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
목 11시 : 30분
유기화학 - KCS-RSC joint symposium- Chemical Biology
저자 및
고려대학교 화학과, Korea
Optical imaging with fluorescence microscopy is a vital tool in the study of living systems. The most common method for cell imaging, one-photon microscopy (OPM), uses a single photon of higher energy to excite the fluorophore. However, two-photon microscopy (TPM), which uses two photons of lower energy as the excitation source, is growing in popularity among biologists because of several distinct advantages. Using TPM, researchers can image intact tissue for a long period of time with minimum interference from tissue preparation artifacts, self-absorption, autofluorescence, photobleaching, and photodamage. However, to make TPM a more versatile tool in biology, researchers need a wider variety of two-photon probes for specific applications. In this context, we have developed a series of two-photon probes for the metal ions, acidic vesicles, membrane, and two-photon glucose tracers having 2-acetyl-6-aminonaphthalene and its derivatives as the fluorophore and receptors for the target ions or membrane or glucose, by considering following requirements: significant two-photon cross section for the bright image, receptors (sensing moiety) that trigger the emission of the two photon excited fluorescence upon binding with the ions or membrane in the living system, sensitivity to the polarity of the environment for the selective detection of cytosolic and membrane-bound probes, appreciable water solubility to stain the cells and tissues, cell permeability, high photostability for a long term imaging. In this seminar, I will briefly describe our recent results on two-photon probes for metal ions, thiols, cancer cells, pH, and their applications in biology and medicine.