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제114회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Characterization of biodegraded petroleum by comprehensive 2D gas chromatography and FT-ICR mass spectrometry

등록일
2014년 8월 28일 14시 26분 48초
접수번호
0984
발표코드
ANAL.P-541 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
10월 15일 (수요일) 16:00~19:00
발표형식
포스터
발표분야
분석화학
저자 및
공동저자
진장미, 김영환*
한국기초과학지원연구원 질량분석연구부, Korea
The bioremediation method is one of the primary ways for degradation of oil spill pollutants by microorganisms. The Pseudomonas taeanensis sp. nov. MS-3 (P.taeanensis MS-3), isolated from a crude oil contaminated seashore in South Korea, has been reported as oil-degrading bacterium. In this study, the P.taeanensis MS-3 was applied to two petroleum derivatives and two crude oils. The P.taeanensis MS-3 strain was inoculated into media containing 0.3% of respective oil samples and incubated at 28℃ for 7 days on a rotary shakes at 170 rpm. The oil components from the media were extracted with toluene. The comprehensive two dimensional gas chromatography with flame ionization detector (2D-GC/FID) and atmospheric pressure photo ionization (APPI) coupled with 15T fourier transform ion cyclotron resonance mass spectrometry (15T FT-ICR MS) were employed to compare chemical composition changes as well as relative degradation rates. As a result, the alkane and ring compounds were mainly detected in extracts by 15T FT-ICR MS. The 2D-GC/FID analysis showed that the percentages of degradation for diesel, kerosene, basrah crude oil and murban crude oil are approximately 11.9 %, 31.3 %, 41.5 % and 53.4 % respectively.

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