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  • 09월 04일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제114회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Light Emitting Diodes as a Photo Transducer in Spectrophotometry

등록일
2014년 8월 28일 14시 31분 54초
접수번호
0993
발표코드
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발표시간
10월 15일 (수요일) 16:00~19:00
발표형식
포스터
발표분야
분석화학
저자 및
공동저자
신동용, 엄인용1,*
대구가톨릭대학교 화학과, Korea
1대구가톨릭대학교 생명화학과, Korea

Light emitting diodes (LEDs) can be used as a photo transducer in optical measurements (i.e. absorbance and fluorescence measurements) even though they have been mostly used so far as a solid state light source in analytical chemistry. Generally speaking, a LED is sensitive to the same wavelength of light which the LED emits. Recently, Shin et al. used a pair of LEDs to be used in absorbance measurement using this principle; a red LED was a light source and the other red LED was used as a photo transducer. However, it is well known that different photo sensors showed different sensitivity to different wavelength. Therefore, It still needs to characterize LEDs in terms of relative sensitivity to the same range of wavelength (especially, IR to visible region). In this report, two pairs of LEDs (i.e. red-red LED and blue-green LED pairs) were used to analyze the mixture of two common indicator dyes (i.e. bromothymol blue and methyl orange) based on these LED characterization data. The green LED was used instead of a blue LED due to the fact that a longer wavelength emitting LED showed relatively higher sensitivity if two LEDs are sensitive somewhat to the same wavelength. Next, a red LED was used to register fluorescence of Rhodamine B which was excited by the illumination of a red LED. There was no need to use an additional optical filter in front of the detector LED due to the LED’s intrinsic optical filtering capability. A calibration curve was obtained for the concentration range of 0.1 ? 10 μM.


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